The Iowa Public Health Department said in a news release Friday that the victim was 81 or older and lived in central Iowa.
The West Nile Virus is spread to humans through the bite of an infected mosquito, according to health officials.
Prince George's County announced its first human case of the virus on Wednesday.
"These traps are tested weekly from June to September, and with this recent confirmation of West Nile virus activity, we are increasing our efforts in the area surrounding the positive traps by looking for breeding sites and re-treating catch basins with larvicide". The virus may also be transmitted through contact with other infected animals, their blood, or other tissues.
Symptoms of West Nile Virus include fever, headache, body aches, nausea, vomiting, swollen lymph glands, a rash on the chest, stomach or back, and a fever.
In Maryland, there were five reported cases of West Nile previous year, according to the Centers for Disease Control and Prevention.
"Mosquitoes are weak flyers and won't travel far from where they are born - if there are mosquitoes in your backyard or neighborhood, they are likely laying eggs there as well", said Matt Helwig, a biolgist with the DEP mosquito program. Less than one percent of those infected develop serious illness of the nervous system like encephalitis or meningitis.
To reduce the number of mosquitoes in your yard, be sure to remove any standing water from items such as pots, hot tubs, pools, buckets or wheelbarrows.
People 60 and older or people with medical conditions are at the greatest risk.