It also stopped her joining Court, Evonne Goolagong and Kim Clijsters as the only mothers to win Grand Slam titles in the professional era. This marks Williams' first Wimbledon loss since 2008 when her sister Venus Williams won the title.
Many expected Serena Williams to claim her 24th Slam but it was Angelique Kerber who left with the trophy on Saturday afternoon.
As Williams said after her semi-final victory against Julia Goerges, there was nothing normal about her being in a 30th grand slam singles final 10 months after giving birth to daughter Olympia and suffering life-threatening complications.
Williams played in a Wimbledon final on Saturday in just her second major back after having a baby in September.
"I'd just like to tell all the moms, I had such a long struggle to come back and it was really hard", Williams explained in her post-match interview.
"It was obviously disappointing but I can't be disappointed, I have so much to look forward to".
Kerber is the first German woman to win the Wimbledon title since Steffi Graf in 1996.
"To all the moms out there, I was playing out there for you today and I tried", Williams said.
Williams battled it ou with 11th-seeded Angelique Kerber. "I think that was part of what I had to learn after 2016, a process I had to go through".
Watch her interview below.
Kerber beats Williams to claim maiden Wimbledon title
Williams was gunning for her eighth title at the All England Club but Kerber dominated the match to flip the tables following the result during the pair's previous showdown.
Emotional after clinching her third Slam, the German bounded up into the players' box to embrace her family and coaching team.
Williams struck back in the fourth game to level up at before rocketing down the fastest serve of the tournament, a 201kph ace, to hit the front for the first - and only - time. "I can, you know, come out and be a contender to win Grand Slams".
Now she's dropped another.
"It's good to just continue that path and just continue to keep going for me".
But it was all about Kerber as she absorbed everything Williams threw at her and counter-punched in very effective fashion.
"Everything has changed the way I thought about raising a daughter", she told Elle in May.
After winning her semifinal against Jelena Ostapenko 6-3, 6-3, Kerber was asked what she sees across the net when she goes up against Williams.
Kerber continued her momentum in the second set, scurrying along the baseline, this way and that, using a combination of quickness and anticipation to track down what often appeared to be winners for Williams but were not enough to end a point.